Assessment of DNA Strand Breakage in Streptozotocin-induced Diabetic Rats

Authors

  • Siti Balkis Budin Universiti Kebangsaan Malaysia
  • Norfadilah Rejab Universiti Kebangsaan Malaysia
  • Khairul Osman Universiti Kebangsaan Malaysia
  • Jamaludin Mohamed Universiti Kebangsaan Malaysia
  • Abdul Gapor Mohd Top Lembaga Minyak Sawit Malaysia (MPOB),
  • Wan Nazaimoon Wan Mohamud Institute of Medical Research (IMR)
  • Mokhtar Abu Bakar Universiti Kebangsaan Malaysia

Keywords:

diabetes mellitus, hyperglycemia, oxidative stress, DNA damage, Alkaline Comet Assay

Abstract

This study was conducted to evaluate the oxidative damage in diabetic mellitus induced rats. The evaluation of DNA damage was carried out by the Alkaline Comet Assay using peripheral lymphocyte cells taken from streptozotocin-induced diabetic rats (50 mg/kg) and control rats. The levels of malondealdehyde (MDA), 4-hydroxynonenal (4-HNE), fasting blood glucose (FBG) and HbA1c were also measured. All the induced diabetic rats were hyperglycemic until the end of the study with significantly higher levels of FBG and HbA1c as compared to the control rats. The results showed the percentage of tail DNA and tail moment values were also significantly higher in the diabetic induced rats. The same observations were made on the levels of plasma MDA and 4-HNE. In conclusion, this study indicated that hyperglycemic condition in diabetic induced rats could generate oxidative DNA damage.

Author Biographies

Siti Balkis Budin, Universiti Kebangsaan Malaysia

Biomedical Sciences DepartmentFaculty of Allied Health Sciences

Norfadilah Rejab, Universiti Kebangsaan Malaysia

Biomedical Sciences DepartmentFaculty of Allied Health Sciences

Khairul Osman, Universiti Kebangsaan Malaysia

Biomedical Sciences DepartmentFaculty of Allied Health Sciences

Jamaludin Mohamed, Universiti Kebangsaan Malaysia

Biomedical Sciences DepartmentFaculty of Allied Health Sciences

Abdul Gapor Mohd Top, Lembaga Minyak Sawit Malaysia (MPOB),

Unit Produk Agro

Wan Nazaimoon Wan Mohamud, Institute of Medical Research (IMR)

Unit Diabetes dan EndokrinPusat Penyelidikan Kardiovaskular, Diabetes dan Pemakanan,

Mokhtar Abu Bakar, Universiti Kebangsaan Malaysia

Pathology Department,Faculty of Medicine

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Published

2005-06-30

Issue

Vol. 3 No. 1 (2005)

Section

Articles

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