Effects of Lacticaseibacillus paracasei Shirota Supplementation on Growth Performance, Intestinal Health, and Fecal AFB1 Metabolite in AFB1-Exposed Rats

Authors

  • Muhammad Firdhaus Shaharudin Department of Nutrition, Faculty of Medicine and Health Science, Universiti Putra Malaysia
  • Siti Raihanah Shafie Department of Nutrition, Faculty of Medicine and Health Science, Universiti Putra Malaysia
  • Maizaton Atmadini Abdullah Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 43400 UPM Serdang, Selangor Malaysia
  • Fauzah Abd Ghani Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 43400 UPM Serdang, Selangor Malaysia
  • Mohd Redzwan Sabran Department of Nutrition, Faculty of Medicine and Health Science, Universiti Putra Malaysia; Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400, Serdang, Selangor

Keywords:

Aflatoxin B1, Probiotics, Lacticaseibacillus paracasei Shirota, Intestinal health

Abstract

Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi.  Finding in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lactobacillus casei Shirota (LcS) supplementation on the growth performance, intestinal health, and excretion of fecal AFB1 metabolite of AFB1-exposed rats. Thirty-two male Sprague Dawley rats were divided into control, AFB1, AFB1+LcS and LcS groups. AFB1 was given at a complete dosage of 25 µg AFB1/kg body weight, while LcS supplementation at 2×109 CFU/mL per day for four weeks. The average body weight of the AFB1 group showed no significant increase from week 2 to 4, while other groups had an increment throughout the study. Besides, AFB1 and AFB1+LcS groups had significantly reduced food intake throughout the treatment. AFB1 exposure caused several changes in the histomorphometry parameters but was normalized with LcS supplementation. Besides, all intestinal parts of the AFB1 groups showed mild to moderate inflammation while only mild inflammation was observed in the jejunum and ileum of the AFB1+LcS group. Fecal Bifidobacterium spp. counts showed an increment in three groups, while the AFB1 group showed a significant reduction. In addition, the fecal AFB1 in the AFB1 group was significantly lower than in the AFB1+LcS group. In conclusion, AFB1 affected growth performance and intestinal health, and wherein the effect was alleviated by LcS supplementation. Further investigation on intestinal permeability and serum and urinary AFB1 level is suggested to understand the mechanism of probiotic-AFB1 interaction in alleviating AFB1 toxicity.DOI : http://dx.doi.org/10.17576/JSKM-2024-2202-05

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Published

2024-07-05

Issue

Vol. 22 No. 2 (2024)

Section

Nutrition & Dietetics

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