Identification of Gram positive and negative bacteria microflora isolated from hot water spring in Bentong, Pahang

Abstract

A study on the isolation and identification of bacterial flora from hot spring was carried out  from Bentong hot spring water, Pahang. In general, many of the bacteria identified from the hot spring were from Bacillus sp. due to its ability to adapt to high temperature mainly by forming endospore. The objective of this study was to identify bacteria other than Bacillus sp. that were able to survive this hot spring. Three hot spring pools were identified as A,B and C.  The temperatures were ranging from 36°C to 52°C and their pH values were between 7.88-8.65. The result shows that two species of Gram positive cocci and three species of Gram negatives were isolated. The two Gram positives were Streptococcus sp. and Staphylococcus sp. The three species of Gram negative rods were identified as Pseudomonas sp., Klebsiella sp. and Proteus sp. In conclusion, non-sporulating species of bacteria other than Bacillus sp. have adapted to survive in hot springs.

DOI : http://dx.doi.org./10.17576/JSKM-2018-1602-01