Determination of Ergosterol as a Potential Biomarker in Pathogenic Medically Important Fungal Isolates

Authors

  • AHMAD SH. A. LAFI 1- Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, University Technology Malaysia, Johor, MALAYSIA. 2- Center of Desert Studies, University of Anbar, IRAQ
  • JACINTA SANTHANAM Biomedical Science Programme, Faculty of Health Sciences, University Kebangsaan Malaysia, Kuala Lumpur, MALAYSIA.
  • TZAR MOHD NIZAM KHAITHIR Department of Medical Microbiology and Immunology, Medical Centre, University Kebangsaan Malaysia, Kuala Lumpur.
  • NUR FASHYA MUSA Institute Bioproduct of Development, University Technology Malaysia, Johor
  • FAHRUL HUYOP Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, University Teknologi Malaysia, Johor, MALAYSIA.

Keywords:

HPLC, Ergosterol, Biomarker, Pathogenic, Fungus

Abstract

Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic fungal species were determined. Liquid chromatography was used for the detection and quantification of ergosterol extracted from fungal broth cultures. Results showed that ergosterol eluted as a single, well resolved peak in the chromatogram profiles of all tested fungi. Based upon relative amounts of ergosterol produced per fungal mycelial dry weight, three groups of fungal pathogens were identified, namely low ergosterol (Aspergillus niger, Candida albicans and Cryptococcus neoformans at 4.62, 6.29 and 7.08 µg/mg respectively), medium ergosterol (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii and Rhizopus sp. at 9.40, 10.79, 10.82, 11.38, 12.60 and 13.40 µg/mg respectively), and high ergosterol (Candida tropicalis at 22.84 µg/mg), producers. Ergosterol was not detectable in bacterial samples, which were included as controls. This first report on ergosterol detection in major pathogenic fungal species indicates that ergosterol may be used as a biomarker to diagnose invasive fungal infections in clinical samples.DOI : http://dx.doi.org./10.17576/JSKM-2018-1602-03

Author Biographies

AHMAD SH. A. LAFI, 1- Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, University Technology Malaysia, Johor, MALAYSIA. 2- Center of Desert Studies, University of Anbar, IRAQ

Biosciences and Medical Engineering department

JACINTA SANTHANAM, Biomedical Science Programme, Faculty of Health Sciences, University Kebangsaan Malaysia, Kuala Lumpur, MALAYSIA.

Biomedical Science Programme

TZAR MOHD NIZAM KHAITHIR, Department of Medical Microbiology and Immunology, Medical Centre, University Kebangsaan Malaysia, Kuala Lumpur.

Department of Medical Microbiology and Immunology, Medical Centre

NUR FASHYA MUSA, Institute Bioproduct of Development, University Technology Malaysia, Johor

Institute Bioproduct of Development

FAHRUL HUYOP, Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, University Teknologi Malaysia, Johor, MALAYSIA.

Department of Biotechnology and Medical Engineering

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Published

2018-06-05

Issue

Vol. 16 No. 2 (2018)

Section

Microbiology

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